Bufalin-loaded vitamin E succinate-grafted chitosan oligosaccharide/RGD-conjugated TPGS mixed micelles inhibit intraperitoneal metastasis of ovarian cancer

Abstract Background Intraperitoneal metastasis is one of the major causes high mortality rate ovarian cancer. Bufalin (BU) an effective component traditional Chinese medicine Chansu that exerts antitumor effects, including inhibition. In our previous studies, we found BU inhibited migration and invasion cancer cells. However, application limited due to its insolubility, toxicity imprecise targeting. The aim this study was use vitamin E succinate (VES)-grafted chitosan oligosaccharide (CSO)/arginine-glycine-aspartic acid peptide (RGD)-conjugated d-alpha-tocopheryl polyethylene glycol 1000 (TPGS) mixed micelles (VeC/T-RGD MMs) deliver cells inhibit intraperitoneal metastasis. Moreover, reduced by coating it with increase biocompatibility for practical applications. Results BU-loaded VeC/T-RGD MMs (BU@MMs) had average diameter 161 ± 1.4 nm, a zeta potential 4.49 1.54 mV loading efficiency 2.54%. results showed these cell proliferation, induced apoptosis, A2780 SKOV3 Further studies indicated BU@MMs enhanced levels e -cadherin decreased expression N-cadherin, vimentin Snail in vitro. addition, effectively anticancer effect metastatic models. exhibited fewer toxic side effects than BU, indicating better biosafety vivo Conclusions Our show are multifunctional nano-drug delivery system can